AP bio question, quick question

Test Preparation AP Test Preparation
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<p>Alright, so I'm doing lab 5 on cellular respiration, with the respirometers. The experiment is set up to take place in room temperature, and 10 degrees Celsius. Germinating and non germinating peas are put in respiromenters in water baths. As CO2 is produced due to cellular respiration, KOH removes the C02, creating a region of lower pressure, causing water to flow into a pippete connected to the beakers. O2 consumption is proportional to the distance the water travels in the pippette towards the beaker and the peas.</p>

<p>My question is, is the reduction in the rate of cellular respiration in the 10 degree climate due the decreased enzymatic activity, or does it have to with the gas laws (PV=nRT), as in it it becuase we are reducing temperature? I</p>

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<p>help, lab due tom.</p>

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<p>Peas germinating at warm temperatures should consume more oxygen than peas germinating at cold temperatures.</p>

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<p>sorry i didn't fully read your question. it actually has to do with both, kind of.</p>

<p>as long as the heat doesn't denature the enzyme, the more you heat this reaction, the faster the molecules will be moving. the faster they move, the more contact they have with the enzyme, and the faster the reaction rate is. as you cool the temperature, the particle-enzyme contact decreases, which is why reaction rate decreases. </p>

<p>i m pretty sure about this but all i have is chemistry to back me up.</p>

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<p>Oh god, we just did this lab in class and it was a disaster. we didnt have enough time to do it and no one fully understood exactly what they were doing. I'm glad I checked this board since now I know the purpose of the experiment!</p>

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<p>I understand the principle behind the increased rate of the particles moving/ enzymatic acitivity... I just didnt know why we had an entire page of review about PV=nRT if it was as simble as purely enzymatic...</p>

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<p>it's not though</p>

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<p>Any change in volume due to temperature will occur to all three vials -- the germinating peas, the non-germinating peas, and the control with glass beads, marbles, whatever you use. The change is the control vial is used to adjust the change in the other two vials. What you're left with is the change due to difference in reaction rate.</p>