<p>Hi~</p>
<p>I just started studying for Bio 2. This question appeared in TPR Bio. I revised the relevant parts in the book but found no solutions to the question .</p>
<p>So if anyone can help me, I would appreciate it.</p>
<p>The question appears in page 423, question 27. For those who do not have the book, here is a picture of the question</p>
<p>It's the thing with the EcoRI and stuff.</p>
<p>EcoRI and HindIII are two enzymes that will essentially cut the plasmid. The spots on the circle are where they will cut, so the answer is the sizes of the pieces. However, notice that they do not bring up PstI. This means that enzyme PstI is NOT present and there will not be a cut in it’s spot. Therefor that 400 kb piece and that 75 kb piece stay together, and you get a 475 kb piece. The 200 and 300 kb pieces are made by EcoRI’s two cuts, and HindIII’s cut and one of EcoRI’s cuts, respectively.</p>
<p>@oscartrey: ahh I see. Thanks <3</p>
