<p>post questions in this thread, but please try to go your lecture notes or the webcast first to answer them if they are simple ones.</p>
<p>**** 60% of our grade!</p>
<p>*** seriously??! UGH.</p>
<p>Class is out 500 points and the final is worth 300 points…my friend told me like a week ago and I had the same response T-T</p>
<p>“What is the result from the complementation test if ye and pa were mutation in different unlinked genes?”</p>
<p>Did anyone get the answer to that? Fischer asked us that at the end of Lecture 29 but he never gave an answer I don’t think…</p>
<p>well, the final is worth 40% actually cuz 20% of that 60 is MT3 technically…</p>
<p>**** that’s a lot of points…ugh, why do i have to have two finals before this one -_-</p>
<p>@ MechRocket</p>
<p>I went to his office hrs today and somebody asked the same question. The answer is that it would be the same result whether it is linked or unlinked; you would see the wild type phenotype. Hope that helps.</p>
<p>Mech: wouldn’t they always complement? yeye;pa+pa+ x ye+ye+;papa Right? Or am I just totally ****ing up as always.</p>
<p>thank u broskis :)</p>
<p>How would you identify bacteria with a loss of function mutation in the CRP gene? Am I missing something? Didn’t he basically tell us the answer? Wouldn’t it just be using X-gal to make the bacteria blue and then all the ones that are white would be CRP-?</p>
<p>i think if Beta Galactosidase doesn’t get produced even when you add lactose, then you have a non-functional CRP gene. so yeah i think you’re right.</p>
<p>i could be wrong though, i came to that conclusion just by looking at the graph, my memory of this stuff is fuzzy…</p>
<p>Well it was only a few lectures ago 
l34 to be exact. I’m still watching hahaha :(</p>
<p>how do you guys study for bio? do you just webcast it or do you take notes or what? just wondering</p>
<p>why do transposons have to be transcribed before they move? I thought they were just cut and pasted…</p>
<p>i think they have to be transcribed to make transposase which does the cut/pasting</p>
<p>Also, did anybody notice that his 2 practice midterms are like exactly the same? haha hopefully thats what this will be…</p>
<p>agree with flutterfly on that question</p>
<p>fischer’s exams are supposed to be pretty easy</p>
<p>personally i think Forte’s section was the hardest, and he also wrote the hardest questions</p>
<p>does anyone have those sample questions he put up for the Midterm? he took them down</p>
<p>How does GAL4 coordinately activate GAL 1, 7, & 10?</p>
<p>i was a little confused even after watching the webcast. lemme run it by you guys.</p>
<p>-so gal 1, 7, and 10 could be really far apart or not even on the same chromosome.
-there will be the same enhancer sequence upstream of each of these genes
-so 3 SEPARATE gal4 proteins will bind to each of those 3 separate enhancers (that have the same sequence)
-they then separately do their thing by speeding up the binding of tfiid to tfiib, which activates the rna polymerase and then transcription can begin at a high rate</p>
<p>does that sound correct? that is my understanding of this. i’m mostly unsure about the 3 separate enhancer sequences part.</p>
<p>thanks.</p>
<p>Wait, so this test has 300 problems…?.. 3 hrs?</p>