<p>Can anyone in AP bio, or anyone that remembers AP bio accurately, explain the pGLO lab for me? I've already done the prac, but I'm trying to write up Conclusions. I know what happened and why the genetic transformation occured, but... AHHH! I'm kinda confused.. :confused:</p>
<p>Please help!</p>
<p>i dont think that anyone really knows what you're trying to say, .......is it a lab on recombinant DNA? i think i did a lab similar to that, but not pGLO...if you want, IM me about the lab maybe i could help you.</p>
<p>pGLO... oh.. i thought it was a standard AP bio lab. Uhhh, we merged jellyfish DNA that codes for Green Florescent Protien, which was part of a plasmid that also codes for resistance to ampicillin, with E. coli cells/DNA and grew cultures on agar containing ampicillin and arabinose (which removed the inhibitor from the GFP plasmid) to see if the genetic transformation was successful. Because of the operons in the plasmid, if the E. coli was able to grow on the medium containing ampicillin, the gene coding for GFP was necessarily expressed.
(I hope that makes some sense)</p>
<p>See, I know enough to write the background information, but when it comes to the conclusions, I'm not exactly sure what I'm supposed to say...</p>
<p>so the gfp was just a marker that will give the e.coli a green florescent glow if resistence of ampicilin is expressed? i just finished a lab pretty similar to that, except for it was a lil bit more complicated, but yea i know what ur saying. your conclusion depends on what your results were like. if they were like the ideal results (meaning the ecoli did grew in ampicilin, which in turn means that the gene was expressed), then say why this was supposed to happen (because of recombinant dna and how bacterial cells can incoporate other plasmids) also, did u forget to mention restriction enzymes somewhre in there? anyways, and if your results didnt match the ideal, then say why it didnt match. for example, the experiment may have gone wrong because you(or your group) did the procedure wrong, maybe the enzymes were old, maybe because the transformation didnt complete for osme other reason. that is the basis of all my ap lab conclusions, say why the results were like how it was. i hope that helped.</p>
<p>oh and excuse my spelling errors</p>
<p>I'm doing that lab tomorrow! But I can't help you with your conclusion yet, ours is not due until the end of this month. Good luck!</p>
<p>we did that lab as well, but we used something else and it wasnt called that. I really dont remember but i can try to help u</p>
<p>thanks you guys! yeah, our results came out with the transformation efficiency value within the expected range, our E. coli grew on ampicillin and glowed when it was supposed to. It was actually really cool looking under the UV light... really really intense green, small hint of blue... too bad the bacteria is so nasty. Did you guys know the University of Hawaii was the first to splice the jellyfish gene for gfp into the dna of white mice? Just a random tid bit, that's kinda cool tho... Anyway, yeah, that lab was fun, except everyone was being germ-o-phobes, thinking they were all going to get food poisoning (me included!). </p>
<p>SlaveToVices: you're doing pGLO! i'm excited! just don't forget to wash your hands... ;) lol, i have bio right before lunch, so... (ahhh, paranoia. good times) lol, one girl asked our teacher what would happen if she licked the E coli (she has since dropped the class!) she was told that she would end up in the hospital and have a good chance of dying... omg lol</p>
<p>i think i've gotten the conclusion figured out, thanks nguyent! its due tomorrow anyway so i'll just turn it in and hope for the best.</p>
<p>That lab inspired me to make a super bacteria resistant to ampicillin, kanamycin, and chloremphenical. Fun stuff</p>
<p>i don't think my class has done that yet. Were doing the drosophilia lab right now (why are we moving so freaking slow!)</p>
<p>Lol, celebrian25: we did drosophilia with the computer program CD, so it only took us a double block (80 min class period) to complete, rather than two weeks (we're such a slacker AP bio class, my teacher just didn't want to have to get a permit to import fruit flies just for the lab ... lol, there are also a lot of slackers in my class and my teacher thought a good deal of the flies would "get killed" in the sorting.)</p>
<p>JCO: dude, that is awesome... haven't I seen you on a lot of other boards? Some of the UCs perhaps? I think I've read the posts of your stats, which are incredibly awesome, btw. Do you mind if I ask what you're first choice school is?</p>
<p>actually, you could probably lick those cells and nothing would happen. the E coli strains used in freshman/ AP Bio are not pathogenic.</p>