AP Bio Help

Test Preparation
1

<p>I have two questions and I have no idea where to start on them.</p>

<p>1: Using the Restriction Map Data for pAMP, ampicillin resistance plasmid and pKAN, kanamcycin resistance plasmid, and the Restriction Enzymes provided in data sheet #2, design an experiment to produce a recombinant DNA plasmid with both Amp and Kan resistance. Finally, design an explain an experiment to trasnform E.Coli MM 294 with this new recombinant plasmid.</p>

<p>2: Explain both of the following:
a. the mechanism of action of restriction enzymes
b. the different results you would expect if a mutation occured at recognition sites for enzyme Y</p>

2

<p>Well, do you have the restriction map data? You need that to start...</p>

3

<p>yeah, it's on a data table.</p>

4

<p>i really need help on this.</p>

5

<p>I would too but then again I'm not in AP Bio. Try posting it in the High School Life forum, you may get more response.</p>

6

<p>I conducted a lab that is similar to your situation.</p>

<p>Petri Dish #1: DNA-
Petri Dish #2: DNA- w/ AMP
Petri Dish #3: DNA+ w/ AMP
Petri Dish #4: DNA+ w/ AMP & K resistance</p>

<p>Restriction enzymes cut the DNA plasmid at a specific locus to insert new DNA.</p>

7

<p>I'd love to help but unfortunately I was sick throughout the molecular genetics/DNA recombination unit and it's by far my weakest area...sorry!</p>

8

<p>thanks, i'll put it in the other forum.</p>

<p>kchen, is that the answer to mine moreorless?</p>

9

<p>^ Well, that was the answer to my lab, which seems similar to yours (mine was also on E. coli and I was studying about restriction enzymes / plasmids / DNA). So yes, it is the answer to yours, more or less.</p>