<p>I need some homework help. I can't find these answers. If anybody can help me, thanks in advance.</p>
<p>1) It is theorectically possible for a gene from any ogranism to function in any other organism. Why is this possible?
A) All organisms have ribosomes.
B) All organisims have the same genetic code
C) All organisms have tRNA
D) ALl organisms have similar nuclei</p>
<p>2) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends of cutting by restricion enzyme Y. You have a plasmid with a single site for Y, but not for X. YOur strategy should be to
A) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
B) cut the plasmid twice with restrion enzyme Y and ligate the two fragments onto the ends of the human DNA fragments cut with restrion enzyme X.
C) cut the plasmid with enzyme X and then insert the gene into the plasmid.
D) cut the plasmid with restion enzyme X and insert the fragments cut with Y into the plasmid.
E) insert the fragments cut with X directly into the plasmid without cutting the plasmid.</p>
<p>3) All of the following statemnts about probes are true except:
A) Shorter probes adhere to more fragments than do longer probes.
B) They must be produced with the same restion enzyme as the fragments.
C) THey are single-stranded segments of DNA or RNA.
D) In mnay cases, a probe from one organism can be used to locate a homologous DNA segment in another organism.
E) THe probe must be labeled witha radioactive isotope or fluorescet tab.</p>
<p>4) The princiopal problem with inserting an unmodified mammalian gene into the bacterial chromosome, and then getting that gene expressed, is that
A) bacteria translate polycistronic messages only.
B) bacterial DNA is not found in a membrane-enclosed nucleus and is therefore incomplatible with mammalian DNA.
C) bacterial RNA polymerase cannot make RNA complementrary to mammalian DNA.
D) bacteria cannot remove eukaryotic introns.
E) prokaryotes use a different genetic code from that of eukaryotes.</p>
<p>5) A DNA profile is produced by
A) treating slected segments of DNA with restriction enzymes.
B) using a probe to locate specific nucleotide sequences.
C) electrophoreses of restion fragments.
D) A and B.
E) A, B, and C</p>
<p>6) What is the designation for the short arm of a chromosome?
A) l
B) s
C) r
D) p
E) q</p>
<p>7) In the bacterial transformation lab, timing and precision of which step was crucial in allowing the entry of the pasmid into the bacterial cell?
A) addition of cells to calsium chorlide solution
B) addition of plasmid to cell suspension
C) heat shock of cells
D) incubation f inoculated plates</p>
<p>8) Which statement below is true concerning the following section of a DNA molecule:
5' GAATTC 3'
3' CTTAAG 5'?
A) THis section of a DNA molecule represents a palindrome.
B) This section of a DNA molecule would most likely be cut by an effective restrion enzyme beween the A and T.
C) THis section of a DNA molecule would not allow hydrogen bonding between the two strands.
D) This section of a DNA molecule would make a good primer for PCR.</p>
<p>Agan, thanks in advance.</p>